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NaOH titration curve for collagen plug preparation at 1.5 mg/mL final concentration Titration outlines the preparation of 500 μl collagen plugs using 3 mg/mL Rat Tail Collagen type I (RTT) stock, 1 M NaOH (1-8 μl), and cell culture media. Each plug contains 250 μl RTT collagen to achieve a final concentration of 1.5 mg/mL. The volume of NaOH is varied to neutralize the collagen solution, and the remaining volume is adjusted with media to maintain a consistent total plug volume of 500 μl. This titration helps determine the optimal volume of NaOH required for proper gel polymerization at physiological pH. Graphic outline: Workflow for generating cell-mediated contraction of 1.5 mg/mL collagen plug in a 24-well plate.

Journal: STAR Protocols

Article Title: Protocol for preparation and execution of collagen plug contraction assay to measure fibroblast contractility induced by growth factors

doi: 10.1016/j.xpro.2026.104566

Figure Lengend Snippet: NaOH titration curve for collagen plug preparation at 1.5 mg/mL final concentration Titration outlines the preparation of 500 μl collagen plugs using 3 mg/mL Rat Tail Collagen type I (RTT) stock, 1 M NaOH (1-8 μl), and cell culture media. Each plug contains 250 μl RTT collagen to achieve a final concentration of 1.5 mg/mL. The volume of NaOH is varied to neutralize the collagen solution, and the remaining volume is adjusted with media to maintain a consistent total plug volume of 500 μl. This titration helps determine the optimal volume of NaOH required for proper gel polymerization at physiological pH. Graphic outline: Workflow for generating cell-mediated contraction of 1.5 mg/mL collagen plug in a 24-well plate.

Article Snippet: NaOH , Non-substitutable , Sigma-Aldrich (Cat# S8045) , Analytical-grade NaOH pellets from Fisher Scientific, VWR/Avantor, Alfa Aesar, or Millipore Sigma.

Techniques: Titration, Concentration Assay, Cell Culture